Fig. 3.
Fluorescence-activated cell sorting (FACS) analysis of infected erythrocytes labelled with CFDA-SE. a DNA staining with Hoechst of parasites grown in the presence of CFDA-SE labelled erythrocytes allows robust detection of four distinct cellular populations. Data are representative of two independent experiments. b A P. falciparum 3D7 inoculum grown in standard culture conditions at 2 % haematocrit and 2 % parasitaemia was diluted (fourfold serial dilutions), in fresh media containing labelled non-infected erythrocytes for 48 h. Parasites were labelled with Hoechst and fluorescence of i-RBCCFDA-SE quantified by FACS. Y axis represents % of i-RBCCFDA-SE with respect to total number of RBC. Error bars represent SEM for three replicates. Established conditions allow detection of initial parasitaemia as low as 0.002 %