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. 2015 Nov 5;6:217. doi: 10.1186/s13287-015-0208-9

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© Yuan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Implantation of the hASC-HLC–3D-AHAM graft into the CCl4-injured liver. a Gross appearance of the hASC-HLC–3D-AHAM graft in the injured liver at 3 days post implantation. Arrow shows the location of the graft. b, c H&E staining analysis of the graft integrated with the liver tissue. Scale bars: 800 μm b, 50 μm c. Immunofluorescence staining of human nuclei (d), ALB (e), and HNF4α (f) in the hASC-HLC–3D-AHAM graft in the CCl4-injured liver. Scale bar: 50 μm. Negative control using isotype IgG for immunofluorescence (f) (insert). AHAM acellular human amniotic membrane