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. Author manuscript; available in PMC: 2016 Dec 28.
Published in final edited form as: Cancer Lett. 2015 Oct 9;369(2):336–343. doi: 10.1016/j.canlet.2015.10.001

Figure 2.

Figure 2

Proliferation of Lgr5-GFP+ and GFP negative cells from obese (HF) mice 12 h after AOM-injection. Colon samples were fixed in 4% PFA and paraffin embedded. Levels of cell proliferation were measured by the EdU Click-It assay. Data are expressed as percentage of EdU-labeled cells relative to the total number of (A) Lgr5-GFP+ stem cells and (B) non-GFP cells. (C) Representative micrographs for EdU—Alexa-647-stained (pink) proliferating cells, Lgr5-GFP+-Alexa-488-stained (green) stem cells and nuclei (blue). Data represent mean ± SEM (n=3-7 per group). Groups with a p-value <0.05 were considered statistically significant by Bonferroni’s multiple comparisons test following a two-way ANOVA.