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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Mol Immunol. 2015 Jul 29;67(2 0 0):407–415. doi: 10.1016/j.molimm.2015.07.001

Fig. 5.

Fig. 5

Induction of ADCC by ch128.1 and its mutants. The ability of the antibodies to mediate ADCC was measured via the calcein release assay. One or 5 μg/mL of the antibodies specified were incubated with calcein AM-labeled Ramos target cells and human PBMC as effector cells (E:T ratio of 50:1). Calcein release was measured with excitation at 485 nm and emission at 520 nm. *p < 0.05, **p < 0.001 as determined by Student's t-test. Results are representative of three independent experiments using PBMC from different donors. Error bars show standard deviation of samples performed in triplicate in each experiment.