Figure 7.
Effect of integrin beta 1 silencing on MG63 response to surface microstructure and surface chemistry. MG63 cells (WT) or MG63 cells with silenced integrin beta 1 (shITGB1) were plated on TCPS, Ti (PT, SLA), or graphitic carbon-coated (G-PT, G-SLA) substrates and cultured until confluence on TCPS. At confluence, cell number (A), alkaline phosphatase specific activity (B), and levels of secreted osteocalcin (C), osteoprotegerin (D), VEGF (E), and active TGF-β1 (F) were determined. *p<0.05, v. TCPS; %p<0.05, v. PT; $p<0.05, graphitic carbon-coated v. Ti; # p<0.05, v. WT.