Skip to main content
. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: J Invest Dermatol. 2014 Aug 7;135(1):102–109. doi: 10.1038/jid.2014.271

Figure 1. Purification of natural and recombinant Der p 11.

Figure 1

(a) A molecular mass marker (M) and 3 μg of purified rDer p 11 and nDer p 11 were separated by SDS-PAGE under reducing and nonreducing conditions and were silver stained. rDer p 11 bands marked with an arrow were subjected to mass spectrometry analysis. (b) Circular dichroism analysis. The molecular ellipticities (θ) (y axis) at different wavelengths (200–260 nm, x axis) are displayed for rDer p 11 (gray) and nDer p 11 (black).