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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Mol Cell Neurosci. 2006 Apr 19;32(0):82–90. doi: 10.1016/j.mcn.2006.02.004

Fig. 6.

Fig. 6

M1 mutations in SCCMS differentially affect calcium permeability. (A) Whole-cell current traces elicited over a range of holding potentials (−10 mV to +15 mV, with 4 representative traces shown) in HEK-293 cells expressing AChRs bearing mutation βV229F in the presence of 1 mM (left) or 10 mM (right) Ca2+. (B) Current –voltage relationships used to determine reversal potentials. (C) Relative permeability ( PCa/PCs) for wild-type or βV229F AChRs computed using reversal potentials and an extended Goldman– Hodgkin–Huxley equation as described in Experimental methods. The value of PCa/PCs obtained for βV229F AChRs, 1.56 ± 0.41 (n = 6), was significantly greater than that of wild type, 0.37 ± 0.24 (n = 4, P < 0.001, mean ± SD).