Table 3.
AChR type (αβδε) | Expression level (fmol) | EC50 (µM) | Hill coefficient | Normalized response (−nA/fmol |
---|---|---|---|---|
Wild type | 2.3 ± 0.5 | 54 ± 1 | 1.4 ± 0.5 | 800 ± 300 |
βV229F | 1.4 ± 0.4 | 5.5 ± 1* | 1.3 ± 0.5 | 2800 ± 700* |
Values are given as the mean ± SEM. Normalized peak channel activity for individual oocytes was obtained by dividing the ACh-induce current at 300 µM by the fmol of AChR expressed on each oocyte. AChR expression levels, EC50, Hill coefficients and normalized responses were calculated using 7 – 13 oocytes.
P < 0.05.