Table 2. Functional classification of the identified proteins and regulation of their abundance.
| Protein | Gene | Locus tag | Functional classification | Regulation a |
|---|---|---|---|---|
| CARBOHYDRATE METABOLISM | ||||
| glyceraldehyde-3-p dehydrogenase | LBA0698 | Glycolysis / Gluconeogenesis | UP b (2.5) | |
| triosephosphate isomerase | tpiA | LBA0700 | Glycolysis / Gluconeogenesis / Fructose and mannose metabolism | DOWN(-1.8) |
| pyruvate kinase | kpyK | LBA0957 | Glycolysis / Gluconeogenesis / Pyruvate metabolism | UP(1.8) |
| D-lactate dehydrogenase | ldhD | LBA0055 | Pyruvate metabolism | DOWN(-1.7) |
| isomerase | LBA0651 | Pentose phosphate pathway | UP(1.6) | |
| AMINO ACIDS BIOSYNTESIS AND METABOLISM | ||||
| aspartate aminotransferase | LBA0856 | Lysine biosynthesis | UP(1.6) | |
| branched-chain amino acid aminotransferase | ilvE | LBA1341 | Valine, leucine and isoleucine biosynthesis or degradation | UP(1.6) |
| glutamine synthetase | glnA | LBA1501 | Alanine, aspartate and glutamate metabolism / Arginine and proline metabolism | DOWN(-1.6) |
| NUCLEOTIDE METABOLISM | ||||
| ribonucleoside triphosphate reductase | rtpR | LBA0041 | Purine metabolism | DOWN(-1.8) |
| AMINO SUGAR AND NUCLEOTIDE SUGAR METABOLISM | ||||
| bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase | glmU | LBA0219 | DOWN(-1.8) | |
| TRANSPORT SYSTEM | ||||
| glutamine ABC transporter ATP-binding protein | LBA1045 | UP(7.8) | ||
| multiple sugar-binding ABC-transporter ATPase | LBA1645 | DOWN(-2.0) | ||
| TRANSLATION | ||||
| isoleucyl-tRNA synthetase | ileS | LBA0817 | DOWN(-1.8) | |
| seryl-tRNA synthetase | serS | LBA1626 | DOWN(-1.8) | |
| ribosome recycling factor Rrf | LBA1267 | UP(2.8) | ||
| 30S ribosomal protein S1 | rpsA | LBA0968 | UP(2.9) | |
| elongation factor G | fusA | LBA0289 | UP(1.6) | |
| elongation factor P | efp2 | LBA1335 | UP(1.6) | |
| TRANSCRIPTION | ||||
| two-component response regulator | LBA1820 | UP(2.0) | ||
| SIGNAL TRANSDUCTION | ||||
| citrate lyase ligase | citC | LBA0914 | Two-component system | UP(1.6) |
| FOLDING, SORTING AND DEGRADATION | ||||
| ATP-dependent Clp protease, ATP-binding subunit ClpE | clpE | LBA0638 | UPb(5.3) | |
| ATP-dependent Clp protease proteolytic subunit | clpP | LBA0694 | UP(1.6) | |
| ATP-dependent protease ATP-binding subunit HslU | hslU | LBA0985 | UP(1.7) | |
| ATP-dependent protease subunit HslV | hslV | LBA0984 | UP(1.6) | |
| heat shock protein GrpE | grpE | LBA1248 | UP(1.6) | |
| molecular chaperone GroEL | groEL | LBA0406 | UP(1.6) | |
| tRNA-specific 2-thiouridylase MnmA | mnmA | LBA0822 | Sulfur relay system | UP(1.7) |
| OXIDATION-REDUCTION PROCESS | ||||
| oxidoreductase | LBA0182 | UP(1.6) | ||
| trp repressor binding protein | LBA1001 | UP(1.7) | ||
| pyridine mercuric reductase | LBA1220 | UP(2.3) | ||
| MISCELLANEOUS | ||||
| cell division protein FtsZ | ftsZ | LBA0812 | UP(1.6) | |
| NAD-dependent dehydratase | UP(2.2) | |||
| hypothetical protein LBA0987 | LBA0987 | UP(2.1) | ||
| outer surface protein | LBA1239 | spot detected only in 2-DE map of L. acidophilus grown with rutin | ||
aChanges in protein levels are reported as the ratio between the relative spot volume from L. acidophilus grown in the presence of rutin and L. acidophilus grown in control conditions (Vrutin/Vcontrol) for increased proteins and as the negative reciprocal values (-Vcontrol/Vrutin) for decreased proteins. Changes in protein levels >1.5 have been considered significant.
bAverage fold change for proteins contained in more than one spot has been calculated considering the relative spot volume of all the spots containing the same protein and a fold change >1.5 has been considered significant.