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. 2015 Nov 6;10(11):e0141408. doi: 10.1371/journal.pone.0141408

Fig 1. The SDF1α immunoreactivity measured by commercially available kits.

Fig 1

(A) SDF1α (500pg/ml) incubated in PBS for 1 h at 37°C was set at 100% immunoreactivity. Incubation in the presence of DPP4 (25 U/l) resulted in a significantly lower immunoreactivity compared to intact SDF1α (RnD lot 1: 40.5 ± 2.8%; RnD lot 2: 32.7 ± 1.5%; Raybiotech: 27.1 ± 2.4%; Peprotech 32.5 ± 1.2%; *p < 0.05; results ± SEM; n = 5). (B) The immunoreactivity of pure SDF1α (500pg/ml) spiked into serum and incubated for 1 h at 25°C or 37°C was measured with different commercial kits. As the 100% reference, SDF1α spiked into inhibited serum (DPP4-I and Roche protease inhibitor cocktail) was chosen. Ex vivo degradation in serum significantly decreased the immunoreactivity of SDF1α (25°C: RnD lot 1: 54.3 ± 4.1%; RnD lot 2: 31.4 ± 6.4%; Raybiotech: 48.3 ± 5.1%; Peprotech: 62.4 ± 2.3% and 37°C: RnD lot 1: 46.6 ± 0.4%; RnD lot 2: 23.5 ± 1.7%; Raybiotech: 25.9 ± 2.7%; Peprotech 47.8 ± 1.9%; *p < 0.05; results ± SEM; n = 5).