Table 3. A. phagocytophilum proteins with reduced peptide methylation in the ΔOMT mutant identified by iTRAQ.
| Accession Number | Protein ID | Peptides with modifications | Occurrence (Methylated/non-methylated)* | Peptide Ratio ΔOMT:Wt** |
|---|---|---|---|---|
| GI88607441 | Branched-chain alpha-keto acid dehydrogenase subunit E2 | TLSELSK Methyl(S)@6 | 3/2 | 0.6651 |
| GI88607727 | GTP-binding protein TypA | INSQVK Methyl (N)@2 | 2/4 | 0.5106 |
| GI88607117 | Hypothetical protein APH_0406 | NVVLGGMLK Methyl(N)@1 | 5/3 | 0.1773 |
| GI88607879 | Major Surface Protein 4 | VEVEVGYK Methyl(E)@4 | 6/11 | 0.109 |
| VEVEVGYK Methyl(E)@2 | 4/11 | 0.1178 | ||
| GI88607043 | P44-16B Outer membrane protein | TKDTAIANFSME Methyl(S)@6 | 3/4 | 0.4831 |
| GI88607849 | Peprotein translocase subunit SecA | RIDNQLR Methyl (D)@3 | 3/3 | 0.4966 |
| GI88607473 | Phosphoribosylamine-glycine ligase | VLVIGSGGR Methyl(I)@4 | 4/2 | 0.6483 |
| GI88607510 | RNA polymerase sigma factor RpoD | AVLADLR Methyl(D)@5 | 3/3 | 0.6772 |
* Number of occurrences of each methylated and non-methylated peptide.
* *Ratios <1.0 are less abundant in ΔOMT and ratios >1.0 are more abundant in ΔOMT.