Table 1. List of PCR primers used in this study for genotyping, Southern blotting and for analysis of cytokine production by quantitative RT-PCR.
| Application | Primer name | Primer sequence 5’– 3’ |
|---|---|---|
| Genotyping | ATMIN F | TCAGCATCTTCTCCAGAGAGACAG |
| ATMIN R | CACATGTGTACAGCACATTCATTG | |
| ATMIN delta | CTCAGGGTACACATACTATGCTTGC | |
| NBS1 F | CAGGGCGACATGAAAGAAAAC | |
| NBS1 R | AATACAGTGACTCCTGGAGG | |
| NBS1 delta | ATAAGACAGTCACCACTGCG | |
| ATM WT F | GCTGCCATACTTGATCCATG | |
| ATM WT R | TCCGAATTTGCAGGAGTTG | |
| ATM mut F | CTTGGGTGGAGAGGCTATTC | |
| ATM mut R | AGGTGAGATGACAGGAGATC | |
| CD2-cre F | AGATGCCAGGACATCAGGAACCTG | |
| CD2-cre R | ATCAGCCACACCAGACACAGAGATC | |
| Southern blotting | Jβ2 | TGAGAGCTGTCTCCTACTATCGATT |
| Vβ5.1 | GTCCAACAGTTTGATGACTATCAC | |
| Vβ8.2 | CCTCATTCTGGAGTTGGCTACCC | |
| Thy1 F | CCATCCAGCATGAGTTCAGC | |
| Thy1 R | GCATCCAGGATGTGTTCTGA | |
| Dβ2 | GTAGGCACCTGTGGGGAAGAAACT | |
| Quantitative RT-PCR | mEF1α F | GCAAAAACGACCCACCAATG |
| mEF1α R | GGCCTGGATGGTTCAGGATA | |
| IFNγ F | TTCAAGACTTCAAAGAGTCTGAGG | |
| IFNγ R | ATCTGGAGGAACTGGCAAAA | |
| TNFα F | ACCGTCAGCCGATTTGCTAT | |
| TNFα R | TTGACGGCAGAGAGGAGGTT | |
| IL6 F | ACCACGGCCTTCCCTACTTC | |
| IL6 R | TTGGGAGTGGTATCCTCTGTGA | |
| IL17A F | CCTGGCGGCTACAGTGAAG | |
| IL17A R | TTTGGACACGCTGAGCTTTG | |
| Xcl1 F | GGCCATGAGAGCTGTAATTTTTG | |
| Xcl1 R | TGGCTTCTGGATCAGCACAA | |
| Ccl1 F | TGATCCCCCAGCTGTGGTA | |
| Ccl1 R | GTTGAGGCGCAGCTTTCTCTA | |
| Cxcl10 F | GATGACGGGCCAGTGAGAA | |
| Cxcl10 R | GCTCGCAGGGATGATTTCAA | |
| Ccl22 F | TGGTGGCTCTCGTCCTTCTT | |
| Ccl22 R | ACCATAGGGACCTGCATCAGA | |
| Ifitm1 F | GCAGCAAGAGGTGGTTGTACTG | |
| Ifitm1 R | TGGTGGCTGTCGCAGAAG |