Figure 4.
Confocal Microscopy Images of HEK293A cells incubated for three hours with a) PBS only b) G5-NH2 c) G5-NH2-TAMRA1 d) G5-NH2-TAMRA2 e) G5-NH2-TAMRA3 f) G5-NH2-TAMRA4 g) G5-NH2-TAMRA5+ h) G5-NH2-TAMRA1.5avg. TAMRA fluorescence is shown in green. The fluorescence deriving from DAPA-stained cell nuclei is shown in blue. Images were obtained with a 40x oil immersion objective. The same set of image locations is presented in Figure 5 using FLIM. Scale bar is 100 μm.