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Synthesis, isolation, and characterization of G5-NH2-TAMRAn (n = 0, 1, 2, 3, 4, 5+, 1.5avg) samples. a) Stochastic conjugation of TAMRA to G5 PAMAM dendrimer b) Isolation of G5-NH2-TAMRAn employing semi-preparative rp-HPLC c) Reinjection of combined fractions on analytical rp-UPLC to determine purity. n = 1.5avg (black), 0 (red), 1 (orange), 2 (yellow), 3 (green), 4 (blue), and 5+ (purple).
