. Author manuscript; available in PMC: 2015 Nov 6.

Published in final edited form as: Cancer Prev Res (Phila). 2013 Jan 30;6(4):290–298. doi: 10.1158/1940-6207.CAPR-12-0449-T

Table 3.

Effect of dietary SAHA and/or dietary energy restriction on regulatory nodes that affect the mTOR-related signaling in mammary carcinomas

Dietary treatment	Ad Lib Ctl	0.1% SAHA	40%DER	40%DER +0.1%SAHA	40%DER +0.1% SAHA-Release	Overall P
IGF-1R	121 ± 5^a	105 ± 6^ab	93 ± 7^b	95 ± 6^bc	117 ± 5^ac	<0.0001
PI3Kp110	90 ± 5^a	84 ± 3^a	49 ± 3^b	71 ± 5^c	86 ± 6^a	<0.0001
Akt^Ser473 ratio	1.73 ± 0.03^a	1.55 ± 0.01^b	1.39 ± 0.02^c	1.30 ± 0.02^c	1.61 ± 0.02^b	<0.0001
AMPK^Thr172 ratio	0.106 ± 0.006^a	0.182 ± 0.004^b	0.203 ± 0.003^c	0.144 ± 0.004^d	0.116 ± 0.005^a	<0.0001
ACC^Ser79 ratio	1.62 ± 0.04^a	1.91 ± 0.04^b	2.48 ± 0.06^c	2.10 ± 0.08^b	1.50 ± 0.04^a	<0.0001
Raptor^Ser792 ratio	0.131 ± 0.002^a	0.160 ± 0.004^bc	0.168 ± 0.006^b	0.153 ± 0.002^ac	0.141 ± 0.002^a	<0.0001
mTOR^Ser2448 ratio	0.65 ± 0.01^a	0.58 ± 0.04^a	0.27 ± 0.03^b	0.44 ± 0.03^c	0.59 ± 0.04^a	<0.0001
P70S6K^Thr389 ratio	1.51 ± 0.04^a	1.26 ± 0.03^b	0.83 ± 0.05^c	0.98 ± 0.04^c	1.24 ± 0.06^b	<0.0001
4EBP1^Thr37/46 ratio	1.38 ± 0.04^a	1.03 ± 0.06^b	0.95 ± 0.04^b	0.97 ± 0.04^b	1.23 ± 0.06^a	<0.0001

Values are means ± SEM (n=8). Actin-normalized western blot data, which are semi-quantitative estimates of protein expression, were analyzed by Kruskall-Wallis rank test. Different superscripts within the same row are statistically significant among different groups (P < 0.05). Ratio, the ratio of phospho-protein (arbitrary units of optical density) to non-phospho-protein (arbitrary units of optical density). Ad Lib, ad libitum; Ctl, control; Ad Lib, ad libitum; Ctl, control; SAHA, suberoylanilide hydroxamic acid; DER, dietary energy restriction. ACC, acetyl-CoA carboxylase; Akt, protein kinase B; AMPK, adenosine monophosphate-activated protein kinase; 4EBP1, eukaryotic translation initiation factor 4E-binding protein 1; Hif-1α, hypoxia-inducible factor-1α; IGF-1R, insulin growth factor 1 receptor; mTOR, mammalian target of rapamycin; P70S6K, 70-kDa ribosomal protein S6 kinase; PI3Kp110, Phosphoinositide 3-kinase p110; Raptor, regulatory associated protein of mTOR.