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. 2015 Nov 6;10(11):e0142270. doi: 10.1371/journal.pone.0142270

Fig 1. Different vaccination regimes induced different responses to mycobacterial antigens.

Fig 1

Determination of the secretion of IFNγ by whole blood cells, measured by ELISA, from animals vaccinated with BCG at week 0 and challenged with M. bovis at week 12; vaccination and challenge are indicated by arrows on the x axis. Group average of antigen-specific whole blood IFNγ secretion, expressed as OD 450nm, was corrected for background and evaluated at the weeks indicated on the x axis. Animal groups are indicated naïve controls (N.C.) (open rhomboid); SIM BCG/Ad85A (inverted open triangle); BCG e.b. (open triangle); BCG s.c. (open square); SIM BCG/BCG (open circle). Error bars represent the standard error of the mean (SEM). Data were analysed using a using a Kruskal-Wallis test with Dunn’s multiple column post-test comparison. Statistical significance is indicated as follows: * difference between BCG/Ad85A and N.C.; ^ indicates differences between BCG e.b. and N.C.; % indicates differences between BCG s.c. and N.C.; & indicates differences between BCG/BCG and N.C. One symbol indicates ρ<0.05.