Fig 2. The comparative frequency of IFNγ secreting cells responding to mycobacterial antigens does not appear to be affected by different vaccination regimes.

The frequency of IFNγ producers in PBMC, as determined by ELISpot, from animals immunised with BCG at week 0 and challenged with 2000 cfu M. bovis at week 12 was determined as indicated in materials and methods; immunisation and challenge are indicated by arrows on the x axis. IFNγ ELISpot was evaluated at the weeks indicated on the x axis, corrected for background and expressed as spot forming units (SFU) per 10⁶/PBMC. No significant differences in the frequency of IFNγ secreting cells responding to PPD-B, Rv0288 or r85A was detected between the different vaccination regimes after vaccination or after infection. N.C. (open rhomboid); SIM BCG/Ad85A inverted triangle; SIM BCG/BCG (open triangle); BCG e.b. (open square); BCG s.c. (open circle). Error bars represent the standard error of the mean (SEM). Data were analysed using a using a Kruskal-Wallis test with Dunn’s multiple column post-test comparison.