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. 2015 Nov 6;10(11):e0142451. doi: 10.1371/journal.pone.0142451

Fig 2. qRT-PCR analysis of relative expression of cecropin genes from fat body, epidermis, hemocytes after inoculation of M. anisopliae.

Fig 2

Actin was used as an internal control. The mRNA levels of cecropins were highly expressed in 30 h after induction of M. anisopliae in fat body and 24 h in epidermis, Px-cec1, Px-cec2 and Px-cec3 showed maximum expression after 36 h, 30 h, 36 h in hemocytes, respectively. In addition, Px-cec3 depicted more sensitivity to M. anisopliae than Px-cec1 and Px-cec2. Relative expression levels of 6 h was arbitrarily set at 1. Three biological replications (n = 3) were conducted, and the 2-ΔΔCt method was used to measure the relative transcription levels. Means with different number of asterisk are significantly different (P<0.05) (Duncan’s Multiple Range Test) among different time after treated with alive M. anisopliae. *: different with the lowest expression after treated with M. anisopliae; **: significant different with the lowest expression after treated with M. anisopliae; ***: highly significant different with the lowest expression after treated with M. anisopliae.