Table 1. List of Primers and their sequences used in experiment.
Gene name | Sequence(5’-------3’) | function |
---|---|---|
Px-cec-R | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | UPM |
Px-cec-F | CTAATACGAC TCACTATAGGGC | UPM |
Px-cec2-F1 | CAGGTGGAATCCGTTCAA | Px-cec2 3 ′ RACE PCR |
Px-cec2-R2 | TGGAGTTGGCTTGTCCTATC | Px-cec2 5 ′RACE PCR |
Px-cec3-F1 | GCTCCCAGGTGGAAAGGC | Px-cec3 3 ′ RACE PCR |
Px-cec3-R2 | CTCCATCCCGGATGTGTCGTCC | Px-cec3 5 ′RACE PCR |
Actin-qF | TGGCACCACACCTTCTAC | qRT-PCR |
Actin-qR | CATGATCTGGGTCATCTTCT | qRT-PCR |
Px-cec1-qF | GCCAAGGTGGAAGCCGTTTA | qRT-PCR |
Px-cec1- qR | TATAGAAGTGGCTTGTCCGATGA | qRT-PCR |
Px-cec2-qF | TTCGTGTTGGTGGCGGTATTC | qRT-PCR |
Px-cec2-qR | GCAGGTCTAGCGATGGAGTTG | qRT-PCR |
Px-cec3-qF | TACTTCTTCTTCACGGTTGTCG | qRT-PCR |
Px-cec3-qR | CCCAATATGCTGGATGCTTGTC | qRT-PCR |
GFP-F | GGATCCTAATACGACTCACTATAGGAAGGGCGAGGAGCTGTTCACCG | dsGFP |
GFP-R | GGATCCTAATACGACTCACTATAGGCAGCAGGACCATGTGATCGCGC | dsGFP |
Spa-RNAiF | GGATCCTAATACGACTCACTATAGGACGCTGCCTCCAACGCTTCT | RNAi |
Spa-RNAiR | GGATCCTAATACGACTCACTATAGGTCCTCGCATACCAGTCCCT | RNAi |
Dor-RNAiF | GGATCCTAATACGACTCACTATAGGCTGAAGCGAAAGCGACAGAAACC | RNAi |
Dor-RNAiR | GGATCCTAATACGACTCACTATAGGCTGCTGCGCCATAGGAGCCATA | RNAi |
Px-cec1-ExpF | GCGAGATCTAAGCCGTTTAAAAAATTGGA | Recombinant expression |
Px-cec1-ExpR | GCCTCGAGTTTGCCAGTAGGTCTGGCTATAG | Recombinant expression |
Px-cec2-ExpF | GCGAGATCTAAATTGGAGCGAGTGGGACAGC | Recombinant expression |
Px-cec2-ExpR | GCCTCGAGCGGCAGGTCTAGCGATGGAGTT | Recombinant expression |
Px-cec3-ExpF | GCGAGATCTTACTTCTTCTTCACGGTTGTCG | Recombinant expression |
Px-cec3-ExpR | GCCTCGAGTCCCATTATGCTGGATGCTTGT | Recombinant expression |
Spa, Spätzle; Dor: Dorsal; AGATCT, restriction enzyme Bgl II; CTCGAG, restriction enzyme Xho I