Skip to main content
. 2015 Nov 6;10(11):e0142451. doi: 10.1371/journal.pone.0142451

Table 1. List of Primers and their sequences used in experiment.

Gene name Sequence(5’-------3’) function
Px-cec-R CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT UPM
Px-cec-F CTAATACGAC TCACTATAGGGC UPM
Px-cec2-F1 CAGGTGGAATCCGTTCAA Px-cec2 3 ′ RACE PCR
Px-cec2-R2 TGGAGTTGGCTTGTCCTATC Px-cec2 5 ′RACE PCR
Px-cec3-F1 GCTCCCAGGTGGAAAGGC Px-cec3 3 ′ RACE PCR
Px-cec3-R2 CTCCATCCCGGATGTGTCGTCC Px-cec3 5 ′RACE PCR
Actin-qF TGGCACCACACCTTCTAC qRT-PCR
Actin-qR CATGATCTGGGTCATCTTCT qRT-PCR
Px-cec1-qF GCCAAGGTGGAAGCCGTTTA qRT-PCR
Px-cec1- qR TATAGAAGTGGCTTGTCCGATGA qRT-PCR
Px-cec2-qF TTCGTGTTGGTGGCGGTATTC qRT-PCR
Px-cec2-qR GCAGGTCTAGCGATGGAGTTG qRT-PCR
Px-cec3-qF TACTTCTTCTTCACGGTTGTCG qRT-PCR
Px-cec3-qR CCCAATATGCTGGATGCTTGTC qRT-PCR
GFP-F GGATCCTAATACGACTCACTATAGGAAGGGCGAGGAGCTGTTCACCG dsGFP
GFP-R GGATCCTAATACGACTCACTATAGGCAGCAGGACCATGTGATCGCGC dsGFP
Spa-RNAiF GGATCCTAATACGACTCACTATAGGACGCTGCCTCCAACGCTTCT RNAi
Spa-RNAiR GGATCCTAATACGACTCACTATAGGTCCTCGCATACCAGTCCCT RNAi
Dor-RNAiF GGATCCTAATACGACTCACTATAGGCTGAAGCGAAAGCGACAGAAACC RNAi
Dor-RNAiR GGATCCTAATACGACTCACTATAGGCTGCTGCGCCATAGGAGCCATA RNAi
Px-cec1-ExpF GCGAGATCTAAGCCGTTTAAAAAATTGGA Recombinant expression
Px-cec1-ExpR GCCTCGAGTTTGCCAGTAGGTCTGGCTATAG Recombinant expression
Px-cec2-ExpF GCGAGATCTAAATTGGAGCGAGTGGGACAGC Recombinant expression
Px-cec2-ExpR GCCTCGAGCGGCAGGTCTAGCGATGGAGTT Recombinant expression
Px-cec3-ExpF GCGAGATCTTACTTCTTCTTCACGGTTGTCG Recombinant expression
Px-cec3-ExpR GCCTCGAGTCCCATTATGCTGGATGCTTGT Recombinant expression

Spa, Spätzle; Dor: Dorsal; AGATCT, restriction enzyme Bgl II; CTCGAG, restriction enzyme Xho I