Phenotype of cells expressing TLR2 in acne lesions. Two-color immunofluorescence confocal images were obtained for (a) CD14, (b) CD3 (green, first panel of each row), and TLR2 (red, second panel of each row). The two images were then superimposed (third panel of each row). Double-positive cells are shown in yellow. TLR2-positive cells near the follicular bulbar region colocalized with CD14+ macrophages but not with CD3+ T cells. Original magnification, ×630.