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. Author manuscript; available in PMC: 2015 Nov 6.
Published in final edited form as: Cancer Immunol Res. 2013 Jul 31;1(4):229–234. doi: 10.1158/2326-6066.CIR-13-0020

Figure 1. Flow cytometric studies can reproducibly measure frequency of ICOS+ CD4 T-cells.

Figure 1

Peripheral blood from a single individual was drawn on 3 different days and cells were unactivated (control) or activated in vitro prior to 2 different operators performing flow cytometry to evaluate frequency of ICOS+ CD4 T-cells on fresh or frozen samples.