Table 1. Expression values of key mcl-PHA biosynthesis genes under three experimental conditions ^a .

				WG		WFA vs WG
				(Sta vs Exp)		(Exp)
Locus-tag (PPUTLS46_)	Gene symbol	Expression abundance b		Differential expression c		Differential expression c
		RNA	Protein	Rnet	Pnet	Rnet	Pnet
005596	phaI	10.1	- d	5.90	+ e	6.61	+
005601	phaF	12.7	21.5	2.49	2.47	3.91	2.42
005606	phaD	9.7	-	-0.53	-	0.07	-
005611	phaC2	11.3	17.0	-0.17	2.53	0.72	2.05
005616	phaZ	10.6	-	0.1	-	-	-
005621	phaC1	12.1	19.0	0.83	2.04	1.94	1.74
013888	phaG*	13.1	-	2.09	+	-2.27	-
021056	phaJ1*	10.5	17.4	-	-	0.46	0.03
006576	phaJ4	11.4	-	0.79	+	0.94	+

^a Exponential versus stationary growth phase in waste glycerol versus exponential growth phase in waste free fatty acids

^b Log2 expression abundance of gene products (mRNA and Protein) of P. putida LS46 grown under mid-log phase of waste glycerol culture (non-mcl-PHA synthesis condition). Values were averaged from two biological replicates

^c Differential expression values (see details in the Materials and Method section) of gene products under the specific comparing conditions: Sta, Stationary phase; Exp, Exponential phase; Significantly up and/or down-regulated proteins are indicated in bold

^d a “-” symbol means not detected mRNA and/or protein expression under mid-log phase of WG culture and/or two tested mcl-PHA synthesis condition(s)

^e a “+” symbol means protein expression of the gene was detectable under the specific mcl-PHA synthesis conditions: stationary culture of WG and/or exponential culture of WFA, respectively (e.g. protein expression abundance of PhaI: 22.9 and 23.5; PhaG: 17.1 under stationary culture of WG; PhaJ4: 19.1 and 18.9)

* symbol indicates proteins (if detected) with S/N (signal to noise) ratio less than 2.8, and thus having FDR greater than 10%.