Fig 2. Detection of mucosal antigen-specific IgG and IgA responses in the vagina.

Mice were vaccinated three times and vaginal lavage samples were taken 1 week after the last vaccination and tested for antigen-specific IgG (a) and IgA (b) by ELISA. Results are expressed as specific antibody (ng)/total antibody (μg). One-way ANOVA with Tukey’s post test was used to analyse data. * P <0.05, ** P < 0.01, *** P < 0.001.