Fig 5. DNA vaccination induces potent IFNγ responses to HIV-1 Env peptides.

Animals were vaccinated three times by the routes indicated. One week after the final vaccination splenocytes were cultured with HIV Env-specific peptide pools, and IFNγ-producing cells were measured using commercial ELIspot kits. Negative responses were assigned a value of 1 to allowing plotting on a log scale. One-way ANOVA with Tukey’s post test was used to analyse data * P <0.05, ** P < 0.01, *** P < 0.001.