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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Alcohol. 2015 Aug 14;49(7):713–720. doi: 10.1016/j.alcohol.2015.06.006

Figure 4. Flow cytometry analysis of neutrophils in lung isolates.

Figure 4

Lung tissue was obtained 24 hours post-injury from all treatment groups. Tissue was digested into a single cell suspension and cells were analyzed by flow cytometry. A) Representative gating for Ly-6G+CD11b+ neutrophils (box) of CD45+ cells (data not shown) from all four treatment groups. B) Absolute number of neutrophils in lung isolates. * p < 0.05 versus sham groups. Data are presented as mean of total neutrophils from 100,000 lung cells per group ± SEM. N = 3-6 animals per group.