Fig. 2.

Effects of different candidate CP110 phosphorylation sites on centrosome clustering when independently challenged with a CDK2 inhibitor in murine and human lung cancer cells. (A) Representative examples of normal bipolar anaphase with one centrosome at each end of spindles (upper panel); anaphase with supernumerary centrosomes clustered to form bipolar spindles (middle panel) and anaphase with multiple centrosomes and that also form multipolar spindles (bottom panel) in ED-1 murine lung cancer cells. (B) ED-1 and Hop62 cells were each transfected with an empty vector, a CP110-WT or a CP110-MUT expressing plasmid. Twenty-four hours after transfection, cells were treated with seliciclib (10μM) for 24 hours and fixed and scored for effective centrosome clustering and multipolar anaphases. All experiments were independently replicated at least three times.