Fig 3.

Effects of Ser 170 and Thr 194 CP110 phosphorylation sites on centrosome clustering when independently challenged with a CDK2 inhibitor in murine and human lung cancer cells. (A) ED-1 and (B) Hop62 cells were transfected with (left panel) the CP110-MUT expression vector with indicated phosphorylation sites restored to wild-type residues or (right panel) the CP110-WT expression vector or with a vector having the indicated phosphorylation sites replaced with alanine or aspartic acid. Twenty-four hours after transfection, cells were treated with seliciclib (10μM) for 24 hours and fixed and scored for effective centrosome clustering. All experiments were independently replicated at least three times.