Fig. 5.

Effects of KRAS on expression of cyclin F and CP110 in human and murine lung cancer cells. (A) Respective CP110, cyclin F, CEP76, USP33 and RAS protein levels in murine lung cancer cell lines. (B) Respective CP110, cyclin F, CEP76, USP33 and RAS protein levels in empty vector or KRAS expression vector stably transfected ED-1 cells. (C) LKR13 and Hop62 cells were independently transfected with each of two different KRAS targeting siRNAs or control siRNA. Effects of KRAS knock-down at 48 and 72 hours on CP110 and cyclin F expression. Expression of CP110 and cyclin F protein at each time point was shown using immunoblot analyses and ImageJ quantification. Experiments were independently replicated at least three times. Quantification by ImageJ was achieved by pooling independent experimental results.