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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Alcohol. 2015 Aug 20;49(7):631–637. doi: 10.1016/j.alcohol.2015.08.001

Fig. 2.

Fig. 2

Effect of short-term alcohol exposure for 4 (panels A & B) and 6 days (panels C & D) on TGFα protein expression in the MBH of prepubertal female rats. (A & C) Representative Western immunoblot of TGFα and β-actin proteins in the MBH isolated from control (lanes 1–3) and alcohol-treated (lanes 4–6) animals. (B & D) Densitometric quantitation of all the bands from 2 blots assessing TGFα protein expression in the MBH. These data were normalized to the internal control β-actin protein, and the densitometric units represent the TGFα/β-actin ratio. Note that alcohol-treated animals showed increased TGFα protein expression on day 4 (panel B) and day 6 (panel D) compared with control animals. The respective bars illustrate the mean (± SEM) of an N of 7–8 per group. The mean blood alcohol levels after 4 and 6 days of treatment with the alcohol diet were 188 mg/dL and 210 mg/dL, respectively. **p < 0.01; ***p < 0.001 vs. control. Modified from Srivastava et al., 2011.