Figure 4.

Inhibition of osteosarcoma metastatic growth in lung organ culture by selected hits from MMV400 library. Green fluorescent protein-positive K7M2 osteosarcoma cells were delivered to three BALB/C mice by tail-vein injection, and the lungs were dissected. Two lung sections from each of three animals (in total six lung sections per treatment) were grown in culture media, and metastatic progression was monitored using a fluorescence microscope. 10 μM of the anti-ezrin compound NSC305787 and 30 μM of antimalarial compounds were added to the culture media at day 0 of the culture period. Images were acquired at day 8 and the area of fluorescence signal in each section was quantified. Data represents fold change in fluorescent area at day 8 of drug-treated lung sections as compared to vehicle-treated control and is shown as mean ± standard deviation of total six lung sections from three mice (*; p<0.05 compared to control; using a Student’s unpaired t test).