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Three-dimensional reconstructed ToF-SIMS data of nanoparticles in cells. Red indicates cell, and green indicates nanoparticles. (a) Side view, (b) side view cut halfway through cells, and (c) top view. From previous data using similar sputter conditions, the sputter rate through similar cells was around 10.8 nm/1.25 × 1013 ions/cm2 (Ref. 11). This gives an estimated cell thickness of ∼0.6 μm.
