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. 2015 Oct 13;16(6):695–712. doi: 10.1007/s10162-015-0547-3

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© The Author(s) 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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FIG. 2 — PCR and Southern blot results for five positive ES cell subclones. A PCR genotyping of DNA from the five positive ES cell subclones carrying the Slc44a2 targeting construct (3.3-kb PCR product), subclone 78 that lacked the targeting construct, and +/+ (wild-type) mouse DNA (3.1-kb PCR product) without the construct. B Southern blot showing the presence of the Slc44a2 8.4-kb wild-type and the 6.0-kb recombinant construct in the five positive subclones of clone 29.