FIG. 3.
Deletion schema in transgenic mice showing the primer sets and PCR results used to demonstrate successful deletion of the neo-cassette and Slc44a2 exons 3–10. A, B PCR results for heterozygous +/neo-deleted floxed Slc44a2, +/Neo-Slc44A2, and a wild-type animal +/+ without the construct. C, D PCR results for correct location of floxed exons 3–10 in heterozygous +/Slc44a2 flox and homozygous Slc44a2 flox/Slc44a2 flox, as well as +/+ mice. E, F PCR results for homozygous Slc44a2 Δ/Slc44a2 Δ, heterozygous +/Slc44a2 Δ, and +/+ mice after crossing to C57BL/6 carrying Cre.