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. 2015 Oct 13;16(6):695–712. doi: 10.1007/s10162-015-0547-3

FIG. 3.

FIG. 3

Deletion schema in transgenic mice showing the primer sets and PCR results used to demonstrate successful deletion of the neo-cassette and Slc44a2 exons 3–10. A, B PCR results for heterozygous +/neo-deleted floxed Slc44a2, +/Neo-Slc44A2, and a wild-type animal +/+ without the construct. C, D PCR results for correct location of floxed exons 3–10 in heterozygous +/Slc44a2 flox and homozygous Slc44a2 flox/Slc44a2 flox, as well as +/+ mice. E, F PCR results for homozygous Slc44a2 Δ/Slc44a2 Δ, heterozygous +/Slc44a2 Δ, and +/+ mice after crossing to C57BL/6 carrying Cre.