FIG. 5.

A Structure of the deleted Slc44a2 gene. The open reading frame of this mutant transcript matched the wild-type Slc44a2 transcript through exon 2 and the first amino acid of exon 3, but exhibited a frameshift in exon 11 that encoded alternative residues until a stop codon within exon 14. B Putative 150-amino acid truncated protein deduced from the RT-PCR expressed message. The first 29 amino acids shown in red are identical to the sequence encoded by exons 1–2 and the first amino acid of exon 3. The open reading frame is spliced into exon 11 out-of-frame resulting in an alternate transcript (in black) that fails to match to any known proteins. If the protein was produced and stable, it would have a molecular mass of 17 kDa. As the N-terminal amino acids are conserved in the fusion transcript, immunoprecipitation and detection with the N-terminal antibody should detect the fusion protein if it is produced and stable.