TABLE 1.

Primers used for cloning the targeting region

5′ KO F	TTCCTTGCTCTGCTTTGTAAGTCC	5′ KO R	CTGTTCATTTTGAAAACTTT-	3.1 kb
Ex KO F	AGACCAGACCTGGTGGCACAG	Ex KO R	GGTAGCAGACATTTGGCACTT	1.9 kb
3′ KO F	CCGGGACCGCTTTCAGAA	3′ KO R	GACATTAGGTAAACATCTTCA	2.7 kb

5′ KO F and 5′ KO R amplify the 5′ homologous region; Ex-KO F and Ex KO R amplify the exon 3–10 targeted deletion site; and 3′ KO F and 3′ KO R amplify the 3′ homologous region