TABLE 4.

Primers used for Slc44a2 full-length cDNA sequencing of wild-type and knockout mice

Mouse-specific full-length primers for isoforms P1 and P2
MsP1 CTL2/SLC44A2 F	TCGCGCTGGCTTCGGACTCA
MsP1 CTL2/SLC44A2 R	GCACAGGGCTGGGCATA CAAG
MsP2 CTL2/SLC44A2 F	GCGGGTGGCGGCTGTGTC
MsP2 CTL2/SLC44A2 R	GGATGGCCAAAGTAGGGGTGAGG
Sequencing primers
Forward primers	Location and name
1. ATGGGACGCCTCAGAAATACGA	Ms AK041533 90–111 bp MsCTL2F1
2. ACACTCGCGACTTTGACTATTACA	Ms AK041533 438–461 bp MsCTL2F2
3. GCGATTCCTGGCTGGCATTAT	Ms AK041533 814–834 bp MsCTL2F3
4. CCCCTTGCGGAATGAGAGC	Ms AK041533 1294–1312 bp MsCTL2F4
5. GGTACCACACGGGCTCCTTAG	Ms AK041533 1551–1571 bp MsCTL2F5
6. ATTGTGGGTAGTGTGGGCATCC	Ms AK041533 1880–1901 bp MsCTL2F6
Reverse primers	Location and name
1. AGAAGCTTGCTGCAGACATCCTA	Ms AK041533 2472–2494 bp MsCTL2R1
2. GTCAGGATCGGAACCCAGTAATAA	Ms AK041533 1963–1986 bp MsCTL2R2
3. GCCCGTGTGGTACCTGAGTGCT	AK041533 1543–1564 bp MsCTL2R3
4. CCCTGCTGGCTTCTTTGATG	Ms AK041533 1093–1112 bp MsCTL2R4
5. AAAGGTTTGCTGGGGGTGATA	Ms AK041533 538–558 bp MsCTL2R5
6. CAGGGGGTTGGCACACTTCA	Ms AK041533 278–297 bp MsCTL2R5