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. Author manuscript; available in PMC: 2015 Nov 7.
Published in final edited form as: Biochem J. 2012 Jan 1;441(1):219–226. doi: 10.1042/BJ20111278

Table 1. Characterization of IMPDHs from filamentous fungi.

Data analyzed as described in Materials and Methods. n.a., not applicable. PbIMPDH-B and PcIMPDH-B did not show NAD+ substrate inhibition up to 5 mM. The concentrations of IMP and NAD+ used for IC50 determination for each enzyme is included in the Supporting information.

IC50 (MPA) (μM) kcat (s−1) KM (IMP) (μM) kcat/KM (IMP) (M−1s−1) KM (NAD+) (μM) kcat/KM (NAD) (M−1s−1) Kü (NAD+) (mM)
AnImdA 0.026 ± 0.002 0.74 ± 0.06 10 ± 1 74,000 ± 8,000 170 ± 70 4,400 ± 100 1.5 ± 0.6
PbIMPDH-A 0.43 ± 0.03 0.70 ± 0.08 130 ± 30 5,400 ± 1,200 340 ± 90 2,100 ± 900 4.7 ± 1.1
PbIMPDH-B 27 ± 9 0.41 ± 0.02 1,400 ± 200 300 ± 80 790 ± 140 500 ± 100 n.a.
PcIMPDH-A 0.036 ± 0.004 0.84 ± 0.03 40 ± 6 21,000 ± 2,000 290 ± 60 2900 ± 300 2.4 ± 0.5
PcIMPDH-B 0.06 ± 0.01 0.0075 ± 0.001 600 ± 300 12 ± 3 640 ± 130 11 ± 6 n.a.