Table 1.
Spectrum of GCR events
| Relevant genotype | CanR 5-FOAR mutation rate* | Isolates analyzed | Telomere addition | Deletion/translocation | Ch V left arm duplication (class I, class II) |
|---|---|---|---|---|---|
| WT | 2.04 [± 0.72] × 10−10 (1.0) | 121 | 9 | 1 | 1 (1, 0) |
| sae2Δ | 1.11 [± 0.08] × 10−9 (5.4) | 9 | 1 | 1 | 7 (4, 3) |
| mre11-H125N | 2.70 [± 0.46] × 10−9 (13.2) | 10 | 2 | 0 | 8 (6, 2) |
| rfa1-t33 | 4.20 [± 1.80 ] × 10−8 (205) | 14 | 7 | 7 | 0 (0, 0) |
| rfa1-t33 sae2Δ | 1.86 [± 1.10] × 10−6 (9079) | 10 | 1 | 0 | 9 (9, 0) |
| rfa1-t33 mre11-H125N | 2.75 [± 0.57] × 10−6 (13436) | 10 | 3 | 0 | 7 (3, 4) |
*
Rate of accumulating CanR 5-FOAR progeny. Numbers in brackets indicate standard deviation.
The number in parenthesis is the fold increase relative to wild type.
Class I: Duplication on Ch V and second homology dependent invasion
Class II: Duplication on Ch V only
1
One WT GCR clone contained a point mutation in the CAN1 gene and no discernable mutation at URA3. This clone was able to grow on 5-FOA containing and SC-URA media.
See also Figure S3.