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. Author manuscript; available in PMC: 2016 Nov 5.
Published in final edited form as: Cell. 2015 Nov 5;163(4):907–919. doi: 10.1016/j.cell.2015.10.022

Figure 3. Syt-GFP Reveals the Presence of Vesicle-Like Structures in the PG.

Figure 3

(A) Representative image of the PG from a wandering control larva overexpressing Syt-GFP (phm22>Syt-GFP). The PG is surrounded by a dashed line. Scale bar, 100 µm.

(B) Representative image of the PG from a day 7 (~150 h AEL) IP3R RNAi larva overexpressing Syt-GFP (phm22>Syt-GFP, IP3R RNAi, dicer2). The PG is surrounded by a dashed line. Scale bar, 100 µm.

(C) Confocal image of the PG from a wandering control larva overexpressing Syt-GFP. Scale bar, 10 µm.

(D) Confocal image of the PG from a day 7 (~150 h AEL) IP3R RNAi larva overexpressing Syt-GFP. Scale bar, 10 µm.

(E) Magnified view of the PG cells from day7 (~150 h AEL) IP3R RNAi larvae overexpressing Syt-GPF. Note aggregation of many small vesicle-like structures along the membrane (arrowheads). Scale bars, 10 µm.