Fig. 1.

Quantitation of Burkholderia spp. intracellular replication. RAW264.7 macrophages were infected with the indicated Burkholderia spp. at a MOI of 10. Two hours post infection, kanamycin was added to reduce the growth of extracellular bacteria. Viable bacteria a) Bt and Bo; b) Bp; c) Bm were quantified at the indicated time points using CFU assays. All data shown is representative of two replicates per strain per time point, and performed on two independent days. Data was normalized and averaged by actual MOI