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. 2015 Nov 6;15:398. doi: 10.1186/s12906-015-0924-9

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© Fernando et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Apoptotic morphology detection by Acridine orange-ethidium bromide (AO/EB) fluorescent staining of RD cell line treated with the MEFCA. a-negative control; b- cycloheximide as the positive control (5 mM; 50 μL); c- 20 μg/mL, d- 50 μg/mL of the extract. Green arrows: live cells, greenish yellow: early apoptotic cells, orange red: late apoptotic cells, red: dead cells (some cells are fragmented and become faded). This figure denotes the results of at least 3 independent experiments (Original magnification 40×)