Fig 2. Effect of lpf1 and lpf2 mutants on adhesion of E. coli O104:H4 to differentiated intestinal epithelium.

E. coli O104:H4, ΔlpfA1, ΔlpfA2, ΔlpfA1 ΔlpfA2, and ΔlpfA1 complemented strains’ adhesion was measured in non-polarized Caco-2 cells. All experiments where done with an MOI of 100 and bacteria was recovered and quantified at 3 h post-infection. Results are expressed as (A) percent input and (B) input compared to the wild type strain. Competition of wild type and ΔlpfA1 was done in the same way and seen in (C) as input of CFUs recovered. Error bars indicated SEM of 3 independent experiments each performed with triplicate wells.