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. 2015 Oct 30;10(10):e0141845. doi: 10.1371/journal.pone.0141845

Fig 3. The Effect of lpfA1 and lpfA2 mutation on adhesion to polarized epitheial cell.

Fig 3

CFUs recovered after a 3 h incubation of wild type O104:H4 2011 c3494, ΔlpfA1, ΔlpfA2, ΔlpfA1 ΔlpfA2, and ΔlpfA1 complemented strains were calculated as percent input (A) and input compared to wild type (B). Competition assays of (C) O104:H4 2011 c3494 wild type and ΔlpfA1 and (D) O104:H4 2009 2050 wild type and O104:H4 c3494 ΔlpfA1 are expressed as percent input of CFUs recovered. All experiments were done with an MOI of 100 and bacteria was recovered and quantified at 3 h post-infection. Error bars indicated SEM of 2–3 independent experiments each performed with duplicate or triplicate wells.