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. 2015 Sep 30;182(3):332–345. doi: 10.1111/cei.12659

Figure 4.

Figure 4

Transactivation of interferon regulatory transcription factor (IRF3) in peripheral blood mononuclear cells (PBMC) and production of interferon (IFN)-α and interleukin (IL)-6 in plasma, in unstimulated and in Toll-like receptor (TLR)-3 and TLR-7/-8-stimulated PBMC. (a) Activation of IRF3 was measured by enzyme-linked immunosorbent assay (ELISA) in nuclear extracts of PBMC from 14 controls, 13 patients in relapse and 13 patients in remission. (b) Correlation between plasma IFN-α and IRF3 activation in nuclear extracts of PBMC from 10 patients in relapse (dark grey plots) and 8 patients in remission (light grey plots) using Spearman's rank correlation test. (c) Secretion of IFN-α was measured using ELISA by day 3 in supernatants of cultured PBMC without stimulation (white bars) and after stimulation by Poly I:C (TLR-3 agonist, hatched bars) and by Resiquimod (TLR-7/-8 agonist, squared bars) in 15 controls, 16 patients in relapse and 13 patients in remission; *P < 0·05; ** P < 0·01; n.s. = not significant. (d) Plasma IL-6 production was measured using ELISA in 40 controls, 28 patients in relapse and 23 patients in remission. (e) Secretion of IL-6 was measured using ELISA by day 3 in supernatants of cultured PBMC without stimulation (white bars) and after stimulation by Poly I:C (TLR-3 agonist, hatched bars) and by Resiquimod (TLR-7/-8 agonist, squared bars) in 21 controls, 22 patients in relapse and 15 patients in remission; *P < 0·05; **P < 0·01; ***P < 0·001; n.s. = not significant.