Fig. 5.

Coculture of MM cells in NBM system induces secretion of factors associated with modification of extracellular matrix and immune response. a Changes in cytokine (IL-6, IL-10, TGFβ-1) levels in conditioned media from 7-day coculture of three patients’ MM cells with NBM. Levels are shown as fold-increase of culture system without MM cells (BM). b Proteomics analyses identified factors in NBM/MM coculture supernatant (increased or decreased levels; p < 0.05; n = 3) compared to supernatant samples of BM cultured without MM cells or MM cells cultured alone. A thorough list is included in Additional file 4: Table S2