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Nox2 was induced by IRI. Semi-quantification of Nox2 immunostaining (DAB; Brown) showed increased staining 4 weeks after IRI compared to baseline. Nox2 levels dropped below baseline levels 6 months after IRI (n=4 for WT and KO at each time point). The Y axis in the right panel is a ratio (no units) of pixels. Specifically, it represents the number of pixels for Nox2 staining divided by number of pixels in the total image.
