Table 2. Significant molecular changes in Nox2−/− vs. wild type mice after IRI.

Effects of Nox2 knockout on molecular signature of IRI. We identified 44 genes involved in fibrosis, oxidative stress, inflammation and response to hypoxia that appear to be associated with the Nox2 response to IRI. The majority of these genes were down-regulated.

(a) Descriptive Table (alphabetical order)
Gene symbol	Fold change	p	Gene symbol	Fold change	p
Inflammatory response (n=15)			Hypoxia (n=12)
Axl	−1.38	0.021	Bhlhe40	−1.41	0.024
Bcl3	−1.40	0.026	Cp	−1.84	0.044
Ccl7	−1.72	0.029	Fgg	−1.68	0.012
Ccr2	−1.62	0.024	Fos	−1.85	0.008
Cfh	−1.54	0.035	Itgb2	−1.34	0.038
Cxcl10	−1.39	0.005	Mmp14	−1.85	0.014
Cxcl3	−1.43	0.031	Ndrg1	1.51	0.014
Ggt5	−1.29	0.007	Nt5e	1.40	0.023
Il34	−1.30	0.032	Plat	−1.39	0.041
Itgb6	1.38	0.004	Sox4	−1.36	0.049
Kit	−1.29	0.020	Tfrc	1.29	0.009
Map3k1	−1.31	0.043	Vcam1	−2.51	0.035
Sdc1	−1.30	0.047
Tlr1	−1.49	0.020
Tlr6	−1.52	0.024
Fibrosis (n=11)			Oxidative Stress (n=6)
Col1a1	−1.79	0.026	Akr1c18	2.18	0.009
Col1a2	−1.58	0.047	Aldh1a1	−1.48	0.024
Col5a1	−1.32	0.013	Apoe	−1.66	0.012
Ctgf	−1.31	0.038	Car3	2.22	0.011
Dcn	−1.43	0.046	Cygb	−1.72	0.037
Fn1	−1.94	0.021	Hspb1	−1.54	0.039
Itga4	−1.35	0.035
Mas1	1.49	0.002
Spp1	−1.53	0.041
Trpv5	−1.36	0.024
Vav3	−1.35	0.027

(b) Upregulation and downregulation of genes after IRI in Nox2−/− compared to wild type mice