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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Surg Res. 2015 Aug 5;199(2):441–449. doi: 10.1016/j.jss.2015.07.042

Figure 3. CYD0682 induces LX-2 cell cycle arrest.

Figure 3

CYD0682 induces S-phase cell cycle arrest (A). LX-2 cells were treated with CYD0682 (0.75 μM) for 24 hours. Cells were stained with propidium iodide and analyzed by flow cytometry as described in Methods. (B) and (C) CYD0682 affects cell cycle regulatory proteins. LX-2 cells were treated with vehicle (0.1% DMSO) or CYD0682 (0.75 μM) for indicated time points. Whole cell lysates were analyzed by Western blot with antibodies for p53, p-p53, and p21. CYD0682 inhibits α-SMA expression (D). Whole cell lysates were analyzed by Western blot with antibodies for α-SMA. GAPDH was used as loading control. The experiments were repeated three times and representative data are shown. α-SMA immunofluorescence (green) is marked reduced following CYD0682 treatment (E). LX-2 cells were treated with CYD0682 (0.75 μM) for 24 hours and stained as described in Methods. DAPI staining was used as a control (blue).