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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Surg Res. 2015 Aug 5;199(2):441–449. doi: 10.1016/j.jss.2015.07.042

Figure 4. CYD0682 promotes LX-2 cell apoptosis.

Figure 4

Apoptosis by CYD0682 was evaluated by Cell Death detection ELISA (each conducted in triplicate) after CYD0682 treatment (at indicated concentrations) for 24 hours. CYD0682 treatment resulted in significant apoptosis (A). Early and late apoptosis were elevated following CYD0682 (0.75 μM) treatment for 24 hours (B). Hoechst 33342 is a nuclear stain used as a control. Yo-Pro-1 is a marker of early apoptosis and propidium iodide is a marker of late apoptosis. Whole cell lysates were analyzed by Western blot with antibodies for procaspase 3 and cleaved-caspase3 (C). LX-2 cells were treated at indicated time points with 0.75 μM of CYD0682. GAPDH was used as a loading control. The results are representative of at least three independent experiments.