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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Surg Res. 2015 Aug 5;199(2):441–449. doi: 10.1016/j.jss.2015.07.042

Figure 6. CYD0682 inhibits TGF-β induced pSmad activity and ECM production.

Figure 6

LX-2 cells were preincubated with CYD0682 (0.75 μM) for 24 hours and then treated with TGF-β (2 ng/mL) for 24 hours, whole cell lysates were analyzed by Western blot with antibodies for fibronectin and type I collagen (A). GAPDH was used as loading control. Nuclear protein levels of phosphorylated-Smad2/3 were analyzed by Western blot (B). Lamin B was used as a loading control. The results are representative of at least three independent experiments.